human total il  (R&D Systems)

Journal: Polymers

Article Title: Electrospun Cellulose Acetate Nanofibers for Healthcare Products: Towards Sensing Pads for Endometriosis

doi: 10.3390/polym18091036

Figure Lengend Snippet: IL-18 ( a ) and TNF-a ( b ) levels after direct contact with different types of electrospun membranes (n = 2 per group). No significant differences were detected through one-way ANOVA ( p > 0.05), indicating the absence of an early inflammatory response.

Article Snippet: After the exposure period, the release of the proinflammatory cytokines interleukin 18 (IL-18) (OriGene Human IL-18 ELISA Kit, OriGene, Rockville, MD, USA) and tumor necrosis factor-alpha (TNF-a) (Abnova TNF-α (human) ELISA Kit, Abnova, Taipei City, Taiwan) into the cell culture supernatant was quantified using an enzyme-linked immunosorbent assay (ELISA), according to the manufacturer’s protocol.

Techniques:

Journal: EJIFCC

Article Title: Plasma Gasdermin D as a Biomarker for Pyroptosis in Early Detection of Newly Diagnosed Type 2 Diabetes Mellitus

doi:

Figure Lengend Snippet: Receiver operating characteristic (ROC) curves of plasma GSDMD, IL-18, IL-1β, and hs-CRP for discrimination of patients with type 2 diabetes mellitus from controls.

Article Snippet: Plasma Gasdermin D (GSDMD) (ELK Biotechnology; Catalog no. ELK-5357), interleukin-18 (IL-18) (Elabscience ; Catalog no. E-EL-H0253), and interleukin-1β (IL-1β) (Elabscience ; Catalog no. E-EL-H0149) were quantified using an ELISA-based platform, with all calibration, quality-control, and validation procedures performed as per the kit manuals ( ).

Techniques: Clinical Proteomics

Journal: iScience

Article Title: An atom-edged magnetic nanomotor for cancer mechanotherapy

doi: 10.1016/j.isci.2026.114994

Figure Lengend Snippet: MagGO primarily induces pyroptosis as the mode of cell death (A–C) CLSM images of CTSB release of U87 (A), MDA-MB-231 (B), and A549 (C) cells after MagGO treatment under 3D MF. The applied field strength is 75 mT. The duration of magnetic field application is 30 min. Scale bars, 20 μm. (D–F) The cell viabilities of U87 (D), MDA-MB-231 (E), and A549 (F) cells treated with MagGO were assessed after pre-treatment with z-VAD-FMK (10 μM), Necrostatin-1 (10 μM), 3-Methyladenine (10 μM), Ferrostatin-1 (2 μM), and MCC950 (10 nM) for 4 h, followed by exposure to 3D MF at 5 Hz for 30 min. The applied field strength was 75 mT. The data were presented as the mean ± SD. Data were analyzed by one-way ANOVA with Tukey’s post hoc test. (G and H) Quantification of IL-1β (G) and IL-18 (H) release from U87 cells for control, MagGO, and MagGO+3D MF ( n = 3). The data were presented as the mean ± SD. Data were analyzed by one-way ANOVA with Tukey’s post-hoc test. (I and J) Western blot analysis of Casp-1 (I) and GSDMD (J) in U87 cells for control, MagGO, and MagGO+3D MF.

Article Snippet: Human IL-18 (Interleukin 18) ELISA Kit , Elabscience , Cat#E-EL-H0253.

Techniques: Control, Western Blot

Journal: Redox Biology

Article Title: Lactate-driven ATP6V1B2 lactylation triggers asthmatic inflammation by linking lysosomal dysfunction to mitochondrial ROS-dependent pyroptosis

doi: 10.1016/j.redox.2026.104059

Figure Lengend Snippet: Identification of cell death type mediated by ATP6V1B2 lactylation and GSDME pathway analysis. (A) GSEA enrichment analysis plot of ATP6V1B2 high-expression samples. (B) Annexin V/7-AAD double staining flow cytometry detecting cell death rate. (C) Cell viability (CCK-8) analysis after treatment with ferroptosis inhibitor (Fer-1) and necroptosis inhibitor (NSA). (D) Cellular morphology observation under bright-field microscope. Scale bar: 100 μm. (E) LDH release rate detection. (F, G) Immunoblot analysis of GSDME (F) and GSDMD/GSDMC (G) cleavage. (H–J) ELISA detection of IL-1β (H), IL-18 (I), and HMGB1 (J) in cell supernatants. LPS + ATP as positive control. (K) Verification of GSDME and GSDMD siRNA knockdown efficiency. (L) Cellular morphology observation after knockdown of GSDME or GSDMD. (M, N) Analysis of LDH release (M) and cell death rate (N) after knockdown of GSDME. Data are expressed as Mean ± SEM. (n = 3 independent experiments) ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant.

Article Snippet: Mouse serum total IgE and HDM-specific IgE (R&D Systems), as well as IL-4 (E-EL-M0043), IL-5 (E-EL-M0722), IL-13 (E-EL-M0727), HMGB1 (E-EL-M0676/E-EL-H1554), IL-1β (E-EL-H0149), and IL-18 (E-EL-H0253) in BALF or cell supernatants were all detected using ELISA kits from Elabscience.

Techniques: Expressing, Double Staining, Flow Cytometry, CCK-8 Assay, Microscopy, Western Blot, Enzyme-linked Immunosorbent Assay, Positive Control, Knockdown

Journal: Science Advances

Article Title: Immunometabolic resistors of aging in long-lived golden spiny mice

doi: 10.1126/sciadv.aec9991

Figure Lengend Snippet: ( A ) The correlation between clusterin expression and maximum life span in various species was analyzed using phylogenetic regression. ( B ) Plasma proteomics of multicohorts of human participants (from 21 to >100 years old) were analyzed by the SomaScan assay. ( C to P ) Eighteen-month-old male C57BL/6N mice were intraperitoneally injected with vehicle (saline) or recombinant mouse clusterin (50 μg/kg) for 2 weeks ( n = 13 per group). (C) Schematic summary for the experiments. (D and E) Health span analyses were conducted. Forelimb grip strength (D) and rotarod (E) tests. (F to I) The representative H&E and Sirius Red staining images with quantification in the gastrocnemius muscle (F), SAT (G), spleen (H), and liver (I). PT, portal vein. Scale bars, 100 μm. (J) Serum IL-1β, IL-6, and tumor necrosis factor–α (TNF-α) levels. (K) Serum monocyte chemoattractant protein-1 (MCP-1) levels. (L) qRT-PCR analysis for chronological age– and mortality-associated genes in the liver ( n = 6 per group). (M to O) Flow cytometry analysis of spleen age-associated B cells (ABCs; M), VAT AABs (N), and VAT M1- or M2-like macrophages (O) (for spleen, n = 13 per group, and for VAT, n = 10 per group). (P) Representative Western blot analysis of liver tissues ( n = 5 per group). ( Q ) Peripheral blood monocytes were isolated from healthy donors and treated with indicated doses of recombinant human clusterin for 1 hour, followed by an indicated duration of lipopolysaccharide (LPS; 1 μg/ml). IL-1β and IL-18 levels in culture supernatants were measured after 24 hours ( n = 5 per group) of LPS treatment. gf, grams-force. FSC-A, forward scatter-A. Data are presented as mean ± SEM. Two-tailed student t test (D to O) or one-way ANOVA with Tukey’s multiple comparisons test (B and Q) was performed. * P < 0.05, ** P < 0.01, and *** P < 0.001. (C): Created in BioRender. V. Dixit (2026); https://BioRender.com/m2sdy6s .

Article Snippet: For culture supernatants of human peripheral blood monocytes, human IL-1β and IL-18 Quantikine ELISA Kits (R&D Systems) were used according to the manufacturer’s instructions.

Techniques: Expressing, Clinical Proteomics, Injection, Saline, Recombinant, Staining, Quantitative RT-PCR, Flow Cytometry, Western Blot, Isolation, Two Tailed Test